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[Phytomitogen-induced lymphocyte transformation - standardization and diagnostic value (author's transl)]
Lymphocytes can transform into blasts. This basic biological property is the prerequisite for many reactions of the cellular immune system. Therefore, lymphocyte transformation tests appear to be a valuable diagnostic approach to define primary and secondary immundeficiency associated with different diseases. The validity of the test system is based on a standardization wich can be achieved sufficiently with regard to culture conditions and measurement of DNA-synthesis. But for phytomitogen-induced lymphocyte transformation a cooperative system of lymphocytes and macrophages is necessary. The results are influenced to a high degree by different cell separation procedures. Therefore, the interpretation of abnormal lymphocyte transformation data, induced unspecifically mitogens, has to take into account alteration of lymphocyte function, different T-/B-cell ratios and changing counts and helper effects of macrophages in order to define a defective lymphocyte transformation more precisely.
Lymphocytes can transform into blasts. This basic biological property is the prerequisite for many reactions of the cellular immune system. Therefore, lymphocyte transformation tests appear to be a valuable diagnostic approach to define primary and secondary immundeficiency associated with different diseases. The validity of the test system is based on a standardization wich can be achieved sufficiently with regard to culture conditions and measurement of DNA-synthesis. But for phytomitogen-induced lymphocyte transformation a cooperative system of lymphocytes and macrophages is necessary. The results are influenced to a high degree by different cell separation procedures. Therefore, the interpretation of abnormal lymphocyte transformation data, induced unspecifically mitogens, has to take into account alteration of lymphocyte function, different T-/B-cell ratios and changing counts and helper effects of macrophages in order to define a defective lymphocyte transformation more precisely.