Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Optical studies on contractile structures during activity.

R L Bourret, Y M Gargouïl, P Régondaud

    Recent Advances in Studies on Cardiac Structure and Metabolism
    |January 1, 1975
    PubMed
    Summary

    This study compares optical properties of myocardial and skeletal fibers, finding differences in scattering and birefringence. Optical changes during contraction precede mechanical events, offering insights into muscle biophysics.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Effect of 2, 4-dinitrophenol at the ventricular level of the mammalian heart].

    Comptes rendus des seances de la Societe de biologie et de ses filiales·2014
    Same author

    [Pharmacodynamics of the human round ligament. Action of acetylcholine, esterine, adrenaline and oxytocin].

    Comptes rendus des seances de la Societe de biologie et de ses filiales·2014
    Same author

    Ultradian variations in sensitivity of rat aorta rings to noradrenaline.

    European journal of pharmacology·1992
    Same author

    Characterization of beta-adrenoceptor subtypes and indications for two cell populations in isolated bovine mesenteric lymphatic vessels.

    European journal of pharmacology·1991
    Same author

    Effects of Daflon 500 mg on bovine vessels contractility.

    International angiology : a journal of the International Union of Angiology·1989
    Same author

    The role of alpha- and beta-adrenoceptors in the response to noradrenaline of lymphatic vessels isolated from the bovine mesentery.

    European journal of pharmacology·1989

    Area of Science:

    • Biophysics
    • Muscle Physiology
    • Optical Spectroscopy

    Background:

    • Myocardial and skeletal muscle fibers exhibit distinct structural and functional characteristics.
    • Understanding their optical properties is crucial for elucidating contraction mechanisms.
    • Previous research has not comprehensively compared these properties during activity.

    Purpose of the Study:

    • To compare the optical properties (scattering, birefringence, absorbance) of myocardial and skeletal fibers at rest and during contraction.
    • To investigate the temporal relationship between optical changes and mechanical contraction.
    • To explore the potential of optical techniques for studying muscle biophysics and biochemistry.

    Main Methods:

    • Comparative analysis of optical properties including scattering and birefringence.
    • In vivo spectrophotometry to record absorbance changes during contraction.
    • Simultaneous recording of optical signals and mechanical activity using a mechanotransducer.
    • Integration of optical data with electrophysiological measurements.

    Main Results:

    • Myocardial trabeculae exhibit greater scattering but less birefringence than skeletal fibers.
    • Absorbance curves reflect the primary components of both fiber types.
    • Increased absorbance at 285 nm during contraction precedes mechanical detection.
    • A spectral shift in absorbance (420-440 nm) suggests early biochemical changes.

    Conclusions:

    • Optical properties differ significantly between myocardial and skeletal muscle fibers.
    • Optical monitoring can detect biochemical and biophysical events preceding muscle contraction.
    • The combined optical and electrophysiological approach offers a powerful tool for studying muscle contraction.

    Related Experiment Videos