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Related Experiment Videos

Comparison of mouse strains using the local lymph node assay.

M R Woolhiser1, A E Munson, B J Meade

  • 1Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505, USA.

Toxicology
|May 18, 2000
PubMed
Summary

The local lymph node assay (LLNA) can identify chemical sensitizers across multiple mouse strains, not just CBA mice. DBA/2, B6C3F1, and BALB/c strains show promise for expanded LLNA use.

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Area of Science:

  • Toxicology
  • Immunology
  • Dermatology

Background:

  • The Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) recommends the local lymph node assay (LLNA) exclusively for CBA mice.
  • Assessing chemical sensitizer responses in diverse mouse strains is crucial for broader toxicological applications.

Purpose of the Study:

  • To evaluate the efficacy of the LLNA in identifying chemical sensitizers across six different mouse strains.
  • To determine if mouse strains beyond CBA can be reliably used in LLNA protocols.

Main Methods:

  • Six mouse strains (C57BL/6, SJL/J, BALB/c, B6C3F1, DBA/2, and CBA) were tested using the LLNA.
  • Alpha-hexylcinnamaldehyde (HCA) was used as a moderate sensitizer, with toluene diisocyanate (TDI) and 2,4-dinitrofluorobenzene (DNFB) as positive controls.

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  • Lymph node cell proliferation was measured as an indicator of sensitization response.

    • Main Results:

    • Lymph node cell proliferation varied significantly across the tested mouse strains following acetone exposure.
    • DBA/2, B6C3F1, BALB/c, and CBA mice exhibited similar proliferation levels across all tested chemicals.
    • All six mouse strains successfully identified the sensitization potential of HCA, TDI, and DNFB, despite differing response magnitudes.

    Conclusions:

    • The LLNA can identify chemical sensitizers across multiple mouse strains, expanding its applicability beyond CBA mice.
    • DBA/2, B6C3F1, and BALB/c mice are suitable candidates for further evaluation as alternative strains for LLNA testing.
    • These findings support the potential for a more inclusive LLNA protocol using a wider range of mouse models.