Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multimicrobial sensor using microstructured three-dimensional electrodes based on silicon technology.

A König1, T Reul, C Harmeling

  • 1Institut für Chemo- und Biosensorik, Münster, Germany.

Analytical Chemistry
|May 18, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Carbon sequestration potential of high-altitude afforestation in the Eastern Central Alps.

Journal of environmental management·2026
Same author

More than rewards: insights into a hospital infection prevention and control gamification strategy.

The Journal of hospital infection·2025
Same author

Inhalation toxicity of thermal transformation products formed from e-cigarette vehicle liquid using an in vitro lung model exposed at the Air-Liquid Interface.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2024
Same author

Endocannabinoid analysis in GlucoEXACT plasma: Method validation and sample handling recommendations.

Talanta·2024
Same author

Metabolomic and lipidomic fingerprints in inflammatory skin diseases - Systemic illumination of atopic dermatitis, hidradenitis suppurativa and plaque psoriasis.

Clinical immunology (Orlando, Fla.)·2024
Same author

Unpacking nudge sensu lato: insights from a scoping review.

The Journal of hospital infection·2023
Same journal

Machine Learning-Assisted Label-Free SERS Decoding of Mitochondrial Molecular Dynamics in Ovarian Granulosa Cells during Aging.

Analytical chemistry·2026
Same journal

Revealing the Regulatory Interplay of NHE1 mRNA and Na<sup>+</sup> in Cancer Cells Using a DNA Nanosensor.

Analytical chemistry·2026
Same journal

Towards Cellular Resolution of Tryptic Peptides in Tissue Sections by MALDI MS Imaging: A Focus on Enzyme Application and Reproducibility.

Analytical chemistry·2026
Same journal

Bioinspired Bilayer Hydrogel Colorimetric Sensor Array for Low-Temperature Food Freshness Analysis.

Analytical chemistry·2026
Same journal

Quartz Crystal Microbalance-Based Point-of-Care Testing Systems: Principles, Device Design, and Applications.

Analytical chemistry·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
See all related articles

This study presents a novel multimicrobial biosensor for environmental monitoring. The sensor accurately detects biochemical oxygen demand (BOD) and polycyclic aromatic hydrocarbons (PAH) in wastewater, offering a versatile and efficient analytical tool.

Area of Science:

  • Environmental Science
  • Biotechnology
  • Sensor Technology

Background:

  • Biosensors offer rapid and sensitive detection of environmental pollutants.
  • Simultaneous analysis of multiple analytes requires sophisticated sensor design.
  • Microbial immobilization techniques are crucial for biosensor stability and performance.

Purpose of the Study:

  • To develop and evaluate a novel multimicrobial biosensor for simultaneous detection of biochemical oxygen demand (BOD) and polycyclic aromatic hydrocarbons (PAH).
  • To assess the performance characteristics and versatility of the developed biosensor for environmental monitoring applications.

Main Methods:

  • Immobilization of two distinct microbial strains (yeast for BOD, PAH-degrading strain) within a single biosensor chip with four platinum electrodes.

Related Experiment Videos

  • Integration of the biosensor chip into a flow-through system for measuring oxygen consumption.
  • Application of chemometrical data analysis for signal interpretation.

    • Main Results:

    • The biosensor demonstrated a low detection limit for naphthalene (0.1 mg/L) and sensor-BOD (1 mg/L), with a calibration range up to 30 mg/L.
    • Achieved precision of 3-6%, a response time of 2-3 minutes, a service life of up to 40 days, and a shelf life of 6 months at 4°C.
    • Successfully analyzed municipal wastewater and PAH-contaminated aqueous samples, showcasing versatility.

    Conclusions:

    • The developed multimicrobial biosensor is simple, mass-producible, and exhibits excellent performance for environmental monitoring.
    • This technology enables simultaneous measurement of sum parameters like BOD and specific pollutants like PAH, providing comprehensive sample analysis.
    • The multimicrobial sensor concept, coupled with chemometrical analysis, paves the way for advanced "electronic tongue" systems for environmental assessment.