Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mitochondria and cell death.

A P Halestrap1, E Doran, J P Gillespie

  • 1Department of Biochemistry, University of Bristol, UK.

Biochemical Society Transactions
|May 18, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biochar effect on sheep feed intake, growth rate and ruminant in vitro and in vivo methane production.

Animal : an international journal of animal bioscience·2024
Same author

The effectiveness of intralesional Candida antigen immunotherapy as a treatment for anogenital warts: a retrospective chart review.

Journal of the European Academy of Dermatology and Venereology : JEADV·2021
Same author

Post-operative pain after laparoscopic right hemicolectomy for Crohn's disease: a case control study.

Acta gastro-enterologica Belgica·2021
Same author

Real-world experience with risankizumab in patients with plaque psoriasis: a retrospective study.

Journal of the European Academy of Dermatology and Venereology : JEADV·2021
Same author

Regional binding of tau and amyloid PET tracers in Down syndrome autopsy brain tissue.

Molecular neurodegeneration·2020
Same author

Knee manipulation for reduced flexion after Total Knee Arthroplasty. Is timing critical?

Journal of clinical orthopaedics and trauma·2018

The study demonstrates that the mitochondrial permeability transition pore (MPTP) opening, triggered by adenine nucleotide translocase (ANT) and cyclophilin-D, causes cell death. Preventing MPTP opening protects against heart injury and influences apoptosis versus necrosis.

Area of Science:

  • Mitochondrial biology
  • Cell death pathways
  • Biochemistry

Background:

  • Mitochondria are central to apoptosis and necrosis via the mitochondrial permeability transition pore (MPTP).
  • MPTP opening is linked to Ca(2+)-triggered conformational changes in adenine nucleotide translocase (ANT) bound to cyclophilin-D.

Purpose of the Study:

  • To directly demonstrate the formation of the MPTP by reconstituting pure components.
  • To investigate the role of MPTP opening and closure in heart ischemia/reperfusion injury.
  • To clarify the MPTP's involvement in different cell death pathways, including apoptosis and necrosis.

Main Methods:

  • Reconstitution of pure adenine nucleotide translocase (ANT) and cyclophilin-D to form the MPTP.
  • Direct observation of MPTP opening in heart ischemia/reperfusion injury.

Related Experiment Videos

  • Analysis of cytochrome c (cyt c) release and mitochondrial depolarization in various apoptotic stimuli.

    • Main Results:

    • Direct reconstitution confirmed ANT and cyclophilin-D form the MPTP.
    • MPTP opening was observed in heart ischemia/reperfusion injury, with recovery correlating to MPTP closure.
    • Transient MPTP opening may facilitate apoptosis by releasing cytochrome c, while closure ensures ATP maintenance for apoptotic cell death.

    Conclusions:

    • The study provides direct evidence for MPTP formation and its critical role in cell death.
    • Modulating MPTP opening and closure presents a therapeutic strategy for ischemia/reperfusion injury.
    • A distinct outer-membrane pathway for cytochrome c release, independent of MPTP, exists and is regulated by Bcl-2 family proteins.