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Angular reconstitution of the Staphylothermus marinus phosphoenolpyruvate synthase
W Li1, F P Ottensmeyer, G Harauz
1Department of Molecular Biology and Genetics, and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
Microscopy Research and Technique
|May 18, 2000
Abstract:
The processes of single particle electron crystallography and three-dimensional angular reconstitution are applied to digital cryoelectron images of a macromolecular complex, the Staphylothermus marinus phosphoenolpyruvate synthase. In particular, the application of IQAD (iterative quaternionic angular determination) is exemplified in the context of more canonical approaches.