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A novel perfusion system for animal cell cultures by two step sequential sedimentation
1Department of Botany, The University of Hong Kong, Hong Kong. zwen@hkusua.hku.hk
Journal of Biotechnology
|May 19, 2000
Summary
A new perfusion system enhances animal cell culture density and monoclonal antibody production. This novel two-step sedimentation method improves separation efficiency for potential industrial applications.
Area of Science:
- Biotechnology
- Cell Culture Engineering
Background:
- High-density animal cell culture is crucial for biopharmaceutical production.
- Conventional perfusion systems face limitations in cell separation efficiency and scale-up.
Purpose of the Study:
- To develop and evaluate a novel perfusion system for high-density animal cell culture.
- To improve cell separation efficiency and monoclonal antibody production.
Main Methods:
- A perfusion system integrating an airlift bioreactor, settling tank, and flat settler was designed.
- A two-step sedimentation process controlled cell flow in a uni-directional manner.
- The system was applied to hybridoma cell cultures.
Main Results:
- Achieved maximum viable cell density of 1.31 x 10^7 cells/mL.
- Reached monoclonal antibody concentration of 400 mg/L and average productivity of 461 mg/L/day.
- Demonstrated significantly higher separation efficiency compared to conventional systems.
Conclusions:
- The developed perfusion system enables high-density animal cell culture with enhanced productivity.
- The two-step sedimentation strategy offers superior cell separation and potential for industrial scale-up.
- This system represents a significant advancement in bioreactor technology for biopharmaceutical manufacturing.
