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Subcellular differences in post-translational modification of barley 14-3-3 proteins
M J van Zeijl1, C Testerink, J W Kijne
1Center for Phytotechnology, Leiden University, The Netherlands Organization for Applied Scientific Research (UL/TNO), TNO Department of Applied Plant Sciences, Wassenaarseweg, AL Leiden, The Netherlands.
Abstract:
Expression and post-translational modification of barley 14-3-3 isoforms, 14-3-3A, 14-3-3B and 14-3-3C, were investigated using isoform-specific antibodies. Although all three isoforms were shown to be present in the cytosolic, the nuclear and the microsomal cell fractions, differences in post-translational modification were identified for the different cell fractions. Germination-related modifications of 14-3-3 proteins were observed in the cytosol and the microsomal fraction, but not in the nucleus. In vitro proteolytic cleavage of 14-3-3 proteins using trypsin suggests that for 14-3-3A this change was caused by proteolytic cleavage of the unconserved C-terminal region.