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Cloning of the Atlantic salmon (Salmo salar) estrogen receptor-alpha gene
S A Rogers1, L Llewellyn, T Wigham
1Cardiff School of Biosciences, Cardiff University, UK.
Abstract:
A cDNA clone encoding most of an Atlantic salmon (Salmo salar) estrogen receptor (ER) was obtained from a liver cDNA library and the remainder of the coding sequence from the gene was isolated from a genomic library. Sequence comparisons showed that the cloned gene represents ER-alpha. Expression of the ER-alpha gene in male and female salmon parr was analysed by RT-PCR. Highest expression was found in brain and liver, with lower levels of ER-alpha mRNA present in all other tissues tested. There was little difference in expression of ER-alpha between male and female.