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Related Concept Videos

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  • Fringe Forms A Complex With Notch.
  • Creative Arts And Writing
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  • Fringe Forms A Complex With Notch.

    • Related Experiment Videos

    Fringe forms a complex with Notch.

    B G Ju1, S Jeong, E Bae

    • 1National Creative Research Initiative Center for Genetic Reprogramming, Institute of Molecular Biology and Genetics, Seoul National University, Korea.

    Nature
    |May 23, 2000

    View abstract on PubMed

    Summary
    This summary is machine-generated.

    Fringe protein modulates Notch signaling by forming complexes with Notch, influencing its ligand preference. This interaction, crucial for development, occurs via specific Notch protein regions and is conserved across species.

    Related Experiment Videos

    Area of Science:

    • Developmental Biology
    • Cell Signaling
    • Molecular Genetics

    Background:

    • Fringe protein and its homologs are key in pattern formation and boundary determination.
    • Fringe is known to modulate Serrate-Notch and Delta-Notch signaling pathways.

    Purpose of the Study:

    • To elucidate the molecular mechanism of Fringe-Notch interaction.
    • To identify the specific domains of Notch involved in binding Fringe.
    • To understand how Fringe binding affects Notch signaling specificity.

    Main Methods:

    • Co-expression of Fringe and Notch proteins.
    • Analysis of Notch mutations (Abruptex59b, AxM1, l(1)N(B)) affecting Fringe interaction.
    • In vitro protein mixing assays.
    • Subcellular colocalization studies.

    Main Results:

    • Fringe and Notch form a complex through Lin-Notch repeats and EGF repeats 22-36 of Notch.
    • Specific mutations in EGF repeats (Ax59b, AxM1) disrupt Fringe-Notch interaction via EGF22-36.
    • A mutation in Lin-Notch repeats (l(1)N(B)) abolishes interaction via Lin-Notch repeats.
    • Ax mutations impact Notch response to Fringe in vivo.
    • Fringe-Notch complex formation may occur prior to secretion.

    Conclusions:

    • Fringe acts cell-autonomously to alter Notch ligand preference by binding specific Notch domains.
    • The conserved Fringe-Notch interaction mechanism underlies its role in diverse developmental processes across species.