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Updated: Aug 8, 2026
The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo
Published on: June 19, 2014
A highly sensitive, non-radioactive assay for T cell activation in cattle: applications in screening for antigens
K T Ballingall1, D M Mwangi, N D MacHugh
1International Livestock Research Institute, P.O. Box 30709, Nairobi, Kenya. k.ballingall@cgiar.org
Abstract:
We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiveness of this assay in detecting rBoIFNgamma and activation of immune CD4(+) and CD8(+) T cell lines and clones in response to specific antigen and transfected COS-7 cells, respectively. We also demonstrate its utility in identifying purified pathogen fractions that activate immune CD4(+) T cell clones.
