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Videomorphometric Analysis of Hypoxic Pulmonary Vasoconstriction of Intra-pulmonary Arteries Using Murine Precision Cut Lung Slices
Published on: January 14, 2014
Specific uptake of 5-hydroxytryptamine is reduced in lungs from hypoxic pulmonary hypertensive rats
T K Jeffery1, L J Bryan-Lluka, J C Wanstall
1Department of Physiology and Pharmacology, The University of Queensland, Queensland 4072, Brisbane, Australia.
Abstract:
In this study, the aim was to determine whether 5-hydroxytryptamine (5-HT) removal by the pulmonary endothelium is reduced in 1-week hypoxic, pulmonary hypertensive rats by directly measuring [3H]5-HT uptake in isolated lungs. In lungs from hypoxic rats, specific 5-HT uptake was reduced. This was due to a 50% decrease in the maximal initial rate of uptake rather than a decrease in affinity of 5-HT for its transporter. It is possible that reduced removal of 5-HT may contribute to the elevation in plasma levels of this vasoactive amine in pulmonary hypertension.
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