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Establishment and characterization of the MSKR inbred strain originated from Japanese wild mice (Mus musculus
A Wada1, Y Kakizoe-Ishida, H Katoh
1Department of Laboratory Animal Science, College of Agriculture, Osaka Prefecture University, Sakai, Japan.
Abstract:
A new inbred strain, MSKR, originated from Japanese wild mice was established in April, 1998. The MSKR mice were 60% of the C57BL/6N inbred mice in the 60-day body weight. Tail length/head-body length and hind-foot length/head-body length of the MSKR mice were significantly smaller than those of the C57BL/6N mice (0.896 vs 1.061, 0.189 vs 0.204), but ear length/head-body length of the MSKR mice was significantly larger than that of the C57BL/6N mice (0.143 vs 0.137). The age of the first parturition and size of the first litter were 63.20 +/- 2.71 days and 6.20 +/- 0.37, respectively, at the 20th and 22nd inbreeding generations. Genetic characterization of the MSKR strain was performed using 34 microsatellite markers, 29 biochemical markers, 9 immunogenetic markers, 3 coat color markers, and mitochondrial DNA RFLP-haplotypes. The result indicated that this newly established inbred strain has some different gene constitution from already known molossinus and common laboratory strains.
Insights
A new MSKR inbred mouse strain, derived from Japanese wild mice, exhibits distinct morphological and genetic characteristics compared to C57BL/6N mice. This novel strain offers a unique genetic resource for research purposes.
Area of Science:
- * Genetics and Genomics
- * Animal Models in Research
Background:
- * Establishment of novel inbred mouse strains is crucial for advancing biomedical research.
- * Characterization of new strains ensures their suitability and distinctiveness for experimental use.
Purpose of the Study:
- * To establish and genetically characterize a new inbred mouse strain, designated MSKR, from Japanese wild mice.
- * To compare the morphological and genetic features of the MSKR strain with the common laboratory strain C57BL/6N.
Main Methods:
- * Morphological measurements including body weight, tail length, hind-foot length, and ear length relative to head-body length.
- * Reproductive parameters such as age of first parturition and litter size.
- * Genetic analysis using microsatellite markers, biochemical markers, immunogenetic markers, coat color markers, and mitochondrial DNA RFLP-haplotypes.
Main Results:
- * MSKR mice showed significantly lower body weight (60% of C57BL/6N), smaller tail and hind-foot lengths, but larger ear length relative to head-body length.
- * Reproductive data indicated first parturition around 63 days with a litter size of approximately 6.2.
- * Genetic characterization revealed a unique gene constitution in MSKR mice compared to known molossinus and laboratory strains.
Conclusions:
- * The MSKR strain represents a novel inbred mouse line with distinct morphological and genetic profiles.
- * Its unique genetic makeup differentiates it from existing laboratory strains, making it a valuable resource.
- * Further research can utilize the MSKR strain for studies requiring specific genetic backgrounds.