Jove
Visualize
Contact Us

Related Experiment Videos

Interactions between hepatitis delta virus proteins.

G Moraleda1, K Dingle, P Biswas

  • 1Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111-2497, USA.

Journal of Virology
|May 24, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

De novo HBV infection caused by an anti-HBc positive donor in a vaccinated liver transplant recipient in spite of anti-HBs response.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2006
Same author

Study on the efficacy and safety of adefovir dipivoxil treatment in post-liver transplant patients with hepatitis B virus infection and lamivudine-resistant hepatitis B virus.

Transplantation proceedings·2006
Same author

Host RNA polymerase requirements for transcription of the human hepatitis delta virus genome.

Journal of virology·2001
Same author

Absence of proximal duct apoptosis in the ventral prostate of transgenic mice carrying the C3(1)-TGF-beta type II dominant negative receptor.

The Prostate·2001
Same author

A stabilized molten globule protein.

FEBS letters·2001
Same author

A single surgeon's experience with the Delaire palatoplasty.

Plastic and reconstructive surgery·2001
Same journal

Dysregulation of miRNAs has broad impacts on virus infection in <i>Drosophila</i>.

Journal of virology·2026
Same journal

Identification of GRP78 as a novel host factor that facilitates zoonotic porcine deltacoronavirus internalization and replication via clathrin-mediated endocytosis.

Journal of virology·2026
Same journal

MGF110-2L deletion prevents IFN-I and inflammatory response, resulting in partial attenuation and protection against virulent ASFV.

Journal of virology·2026
Same journal

Periodic genome sequences facilitate packaging in a single-stranded DNA virus.

Journal of virology·2026
Same journal

A novel monoclonal antibody targeting the hemagglutinin-neuraminidase of peste des petits ruminants virus maintains neutralizing activity by blocking viral adsorption and receptor interaction.

Journal of virology·2026
Same journal

ATG9A is an essential host factor for parechovirus RNA replication.

Journal of virology·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Hepatitis delta virus (HDV) delta antigens interact through specific protein regions, influencing genome replication and particle assembly. Mutations in conserved amino acids confirm these interactions are crucial for HDV

Area of Science:

  • Virology
  • Molecular Biology
  • Structural Biology

Background:

  • Hepatitis delta virus (HDV) delta antigens (deltaAg-S and deltaAg-L) have distinct roles in viral replication and assembly.
  • A shared N-terminal region (aa 12-60) mediates protein-protein interactions essential for HDV lifecycle.
  • Previous structural studies suggested coiled-coil dimer formation with potential for tetramerization.

Purpose of the Study:

  • To investigate if in vitro structural predictions of delta antigen multimerization apply to full-length proteins in vivo.
  • To determine the role of conserved amino acids in delta antigen protein-protein interactions and biological functions.

Main Methods:

  • Site-directed mutagenesis of nine conserved amino acids to alanine in both deltaAg-S and deltaAg-L.

Related Experiment Videos

  • In vivo assessment of mutant deltaAg-S ability to support HDV genome replication.
  • In vivo evaluation of mutant deltaAg-L as dominant-negative inhibitors of HDV replication.

    • Main Results:

    • Mutations in conserved amino acids significantly affected the in vivo functions of both deltaAg-S and deltaAg-L.
    • The observed effects of mutations correlated with the predicted hierarchy of importance for multimerization.
    • DeltaAg-S supported HDV replication, while deltaAg-L inhibited it, with both activities modulated by the mutations.

    Conclusions:

    • Ordered protein-protein interactions mediated by the N-terminal region are critical for delta antigen biological activities.
    • In vivo functions of delta antigens in HDV replication and assembly depend on their multimerization status.
    • Structural predictions of peptide interactions can be extrapolated to full-length proteins in a biological context.