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Translational frameshift sites within bacteriophage lambda genes rexA and cI.
1Department of Microbiology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
Acta Biochimica Polonica
|May 29, 2000
Summary
Bacteriophage lambda
Area of Science:
- Molecular Biology
- Genetics
- Virology
Background:
- The bacteriophage lambda cI-rexA-rexB operon exhibits polarity, a gene expression regulation phenomenon.
- The precise mechanism behind this polarity has remained unexplained.
Purpose of the Study:
- To investigate the regulatory mechanism of the bacteriophage lambda cI-rexA-rexB operon.
- To elucidate the role of translational frameshifting in operon polarity.
Main Methods:
- Identification of potential -1 translational frameshift sites within the operon.
- Development of a model for translational frameshift suppression.
Main Results:
- Three potential -1 translational frameshift sites were identified.
- A model proposing translational frameshift suppression by the lambda CI repressor was developed.
Conclusions:
- Translational frameshift suppression by the CI repressor is proposed as a mechanism for regulating operon polarity.
- This suggests an additional self-regulatory role for the lambda CI repressor.