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Isolation of alpha1-antitrypsin in a new preparative electrophoresis apparatus
The construction and operation of an apparatus is described which enables alpha1-antitrypsin to be isolated from human serum by preparative electrophoresis in polyacrylamide gel. The initial pass through the chamber yields a fraction that is predominantly albumin and several alpha1-proteins. After removal of albumin by affinity chromatography, a second pass through the chamber separates the individual alpha1-proteins. A volume of 50 ml of serum may be accommodated by the chamber, and the recovery of activity in each step is greater than 60%. The entire procedure may be completed in 36 hrs.
The construction and operation of an apparatus is described which enables alpha1-antitrypsin to be isolated from human serum by preparative electrophoresis in polyacrylamide gel. The initial pass through the chamber yields a fraction that is predominantly albumin and several alpha1-proteins. After removal of albumin by affinity chromatography, a second pass through the chamber separates the individual alpha1-proteins. A volume of 50 ml of serum may be accommodated by the chamber, and the recovery of activity in each step is greater than 60%. The entire procedure may be completed in 36 hrs.