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Effect of brefeldin A on Mayaro virus replication in Aedes albopictus and Vero cells
1Institute of Biophysics, C.C.F, Rio de Janeiro, Brazil.
Abstract:
Brefeldin A (BFA), a fungal metabolite that blocks transport of newly synthesized proteins from the endoplasmic reticulum, was found to inhibit Mayaro virus replication. At the concentration of 0.05 microgram/ml, the yield of the virus was inhibited by 94% in Aedes albopictus cells and by 99.5% in Vero cells. Treatment of A. albopictus cells with BFA did not inhibit the virus protein synthesis. However, this compound drastically reduced viral protein synthesis in Vero cells. The inhibitory effect progressively declined when BFA was added at late times post infection (p.i.). The effect of BFA on protein glycosylation is discussed.
Insights
Brefeldin A (BFA), a fungal metabolite, significantly inhibits Mayaro virus replication in cell cultures. This compound disrupts viral protein synthesis, particularly in Vero cells, offering potential antiviral strategies.
Area of Science:
- Virology
- Cell Biology
- Mycology
Background:
- Mayaro virus (MAYV) is an arbovirus causing significant human disease.
- Understanding viral replication mechanisms is crucial for developing antiviral therapies.
- Brefeldin A (BFA) is a fungal metabolite known to disrupt protein transport from the endoplasmic reticulum.
Purpose of the Study:
- To investigate the inhibitory effect of Brefeldin A (BFA) on Mayaro virus replication.
- To determine the impact of BFA on viral protein synthesis and glycosylation.
Main Methods:
- Mayaro virus replication assays were performed in Aedes albopictus and Vero cells.
- Brefeldin A was administered at various concentrations and time points post-infection.
- Viral protein synthesis and potential effects on protein glycosylation were analyzed.
Main Results:
- BFA demonstrated potent inhibition of Mayaro virus yield, with 94% inhibition in A. albopictus cells and 99.5% in Vero cells at 0.05 µg/ml.
- BFA treatment did not inhibit viral protein synthesis in A. albopictus cells but drastically reduced it in Vero cells.
- The inhibitory effect of BFA diminished when added at later stages post-infection.
Conclusions:
- Brefeldin A effectively inhibits Mayaro virus replication in a dose-dependent manner.
- The mechanism of inhibition involves disruption of viral protein synthesis, particularly in Vero cells.
- BFA's impact on protein glycosylation warrants further investigation for potential therapeutic applications.