Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transformation-derived Neisseria gonorrhoeae plasmids with altered structure and function.

T E Sox, W Mohammed, P F Sparling

    Journal of Bacteriology
    |May 1, 1979
    PubMed
    Summary

    Penicillinase plasmids in Neisseria gonorrhoeae can change size after transformation. The study found altered penicillinase (Pcr) plasmids, with a 5.1 kb version potentially arising from a 7.1 kb precursor via deletion.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Short-term Toxicity of High Dose Rate Brachytherapy in Prostate Cancer Patients with Inflammatory Bowel Disease.

    Clinical oncology (Royal College of Radiologists (Great Britain))·2018
    Same author

    Colonic Carcinoma Associated with Malakoplakia.

    The West Indian medical journal·2015
    Same author

    Six-core versus twelve-core prostate biopsy: a retrospective study comparing accuracy, oncological outcomes and safety.

    Irish journal of medical science·2015
    Same author

    The Impact of Molecular Technology on STD Control : A Historical Perspective.

    Methods in molecular medicine·2011
    Same author

    Interstitial cells of Cajal and intestinal function in Trinidadian children.

    The West Indian medical journal·2009
    Same author

    Linear and quadratic dispersion characterization of millimeter-length fibers and waveguides using common-path interferometry.

    Optics letters·2007

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Neisseria gonorrhoeae is a significant human pathogen.
    • Penicillinase production in gonococci is often mediated by plasmids.
    • Understanding plasmid dynamics is crucial for combating antimicrobial resistance.

    Purpose of the Study:

    • To investigate the characteristics of penicillinase (Pcr) plasmids in Neisseria gonorrhoeae transformants.
    • To analyze the size, stability, and mobilization of altered Pcr plasmids.
    • To determine the origin of commonly observed smaller Pcr plasmids.

    Main Methods:

    • Transformation of Neisseria gonorrhoeae with a 7.1 kb Pcr plasmid.
    • Analysis of Pcr plasmid size variation in transformants using gel electrophoresis.

    Related Experiment Videos

  • Assessment of plasmid stability and mobilization frequency.
  • Construction of restriction endonuclease cleavage maps for Pcr plasmids.

    • Main Results:

    • Transformation resulted in penicillinase-producing gonococci with Pcr plasmids of varying sizes (3.45-42 kb).
    • Approximately 25% of transformants contained altered Pcr plasmids, with 5.1 kb being the most common size.
    • Some altered plasmids exhibited differences in stability and mobilization frequency.
    • Restriction mapping revealed the 5.1 kb plasmid's characteristics.

    Conclusions:

    • Transformation of Neisseria gonorrhoeae with Pcr plasmids can lead to significant plasmid size alterations.
    • The 5.1 kb Pcr plasmid appears to be a common derivative, potentially formed through transformation-associated deletions.
    • These findings provide insights into gonococcal plasmid evolution and stability.