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Amperometric biosensors/sequential injection analysis system for simultaneous determination of S- and R-captopril
R I Stefan1, J K van Staden, H Y Aboul-Enein
1Department of Chemistry, University of Pretoria, South Africa.
Abstract:
Two amperometric biosensors based on L- and D-amino acid oxidase, respectively, are proposed for the simultaneous detection of S- and R-captopril in a sequential injection analysis system (SIA). The linear concentration ranges are: 0.4-1.6 micromol/l (S-captopril) and 120-950 nmol/l (R-captopril) with detection limits of 0.2 and 15 nmol/l, respectively. The biosensors/SIA system can be used reliably on-line in synthesis process control, for the simultaneous assay of S- and R-captopril with a frequency of 34 samples/h.
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