Related Experiment Video
Updated: Aug 7, 2026

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
Published on: July 4, 2011
Fluorescence line-narrowing detection in chromatography and electrophoresis
R Jankowiak1, K P Roberts, G J Small
1Department of Chemistry, Iowa State University, Ames 50011, USA. jankowiak@ameslab.gov
Abstract:
A review of the basic aspects of fluorescence line-narrowing spectroscopy (FLNS) and its coupling with thin-layer chromatography (TLC) and polyacrylamide gel electrophoresis (PAGE) for off-line high-resolution low temperature spectral characterization is discussed. This is followed by a description of the on-line interfacing of capillary electrophoresis (CE) and capillary electrochromatography (CEC) with FLN detection. CE/ CEC-FLNS instrumentation and its applications for spectral identification of closely related analytes are also presented. Future prospects of micro and capillary high performance liquid chromatography (HPLC) with on-line high-resolution low temperature spectroscopic identification are considered.
Related Concept Videos
Immunofluorescence Microscopy
The...
Gas Chromatography: Types of Detectors-I
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...
Gas Chromatography: Types of Detectors-II
Photoluminescence: Applications
Fluorescence and Phosphorescence: Instrumentation
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.

