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Inactivation of Listeria monocytogenes Scott A on artificially contaminated frankfurters by high-pressure processing
L A Lucore1, T H Shellhammer, A E Yousef
1Department of Food Science and Technology, The Ohio State University, Columbus 43210, USA.
Abstract:
Vacuum-packaged frankfurters, inoculated with 24-h cultures of Listeria monocytogenes Scott A (approximately 10(9) CFU/ml) by injection into the packages, were held at pressures of 300, 500, and 700 MPa for up to 9 min. L. monocytogenes were washed from the surface of the frankfurter and plated onto brain heart infusion agar. During the time to achieve 300, 500, and 700 MPa (come-up time), L. monocytogenes populations decreased by 1, >3, and >5 logs, respectively. Additional inactivation of L. monocytogenes occurred while the samples were held at 300 and 500 MPa. A 5-log reduction in bacterial population was possible at all pressure treatments; however, pressurization at 700 MPa showed the fastest inactivation with L. monocytogenes reduced from 10(8) to 10(2) CFU/package during the come-up time. These results show that high-pressure processing may be a viable method for controlling foodborne pathogens in postprocessed, packaged frankfurters.
Insights
High-pressure processing effectively inactivates Listeria monocytogenes in vacuum-packaged frankfurters. Even brief pressure application significantly reduces bacterial populations, showing promise for food safety.
Area of Science:
- Food Microbiology
- Food Processing Technology
Background:
- Listeria monocytogenes is a significant foodborne pathogen.
- Post-processing contamination of frankfurters poses a food safety challenge.
Purpose of the Study:
- To evaluate the efficacy of high-pressure processing (HPP) in reducing Listeria monocytogenes in vacuum-packaged frankfurters.
- To determine the impact of different pressure levels and durations on bacterial inactivation.
Main Methods:
- Vacuum-packaged frankfurters were inoculated with Listeria monocytogenes Scott A.
- Samples were subjected to high hydrostatic pressure (HHP) at 300, 500, and 700 MPa for up to 9 minutes.
- Bacterial populations were enumerated after pressure treatment.
Main Results:
- Listeria monocytogenes populations decreased by 1, >3, and >5 logs during the come-up time to 300, 500, and 700 MPa, respectively.
- A 5-log reduction was achievable at all pressure treatments.
- 700 MPa demonstrated the fastest inactivation, reducing bacteria from 10(8) to 10(2) CFU/package during come-up.
Conclusions:
- High-pressure processing is a viable method for controlling foodborne pathogens in postprocessed, packaged frankfurters.
- HPP offers a promising approach to enhance the safety of ready-to-eat meat products.
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