Inactivation of Listeria monocytogenes Scott A on artificially contaminated frankfurters by high-pressure processing

L A Lucore1, T H Shellhammer, A E Yousef

  • 1Department of Food Science and Technology, The Ohio State University, Columbus 43210, USA.

Insights

High-pressure processing effectively inactivates Listeria monocytogenes in vacuum-packaged frankfurters. Even brief pressure application significantly reduces bacterial populations, showing promise for food safety.

Area of Science:

  • Food Microbiology
  • Food Processing Technology

Background:

  • Listeria monocytogenes is a significant foodborne pathogen.
  • Post-processing contamination of frankfurters poses a food safety challenge.

Purpose of the Study:

  • To evaluate the efficacy of high-pressure processing (HPP) in reducing Listeria monocytogenes in vacuum-packaged frankfurters.
  • To determine the impact of different pressure levels and durations on bacterial inactivation.

Main Methods:

  • Vacuum-packaged frankfurters were inoculated with Listeria monocytogenes Scott A.
  • Samples were subjected to high hydrostatic pressure (HHP) at 300, 500, and 700 MPa for up to 9 minutes.
  • Bacterial populations were enumerated after pressure treatment.

Main Results:

  • Listeria monocytogenes populations decreased by 1, >3, and >5 logs during the come-up time to 300, 500, and 700 MPa, respectively.
  • A 5-log reduction was achievable at all pressure treatments.
  • 700 MPa demonstrated the fastest inactivation, reducing bacteria from 10(8) to 10(2) CFU/package during come-up.

Conclusions:

  • High-pressure processing is a viable method for controlling foodborne pathogens in postprocessed, packaged frankfurters.
  • HPP offers a promising approach to enhance the safety of ready-to-eat meat products.

Related Concept Videos

Pasteurization and Food Preservation01:28

Pasteurization and Food Preservation

Pasteurization is a widely employed thermal processing technique designed to enhance the safety and shelf life of perishable food and beverages. By subjecting products to specific high temperatures for controlled durations, this method effectively inactivates pathogenic microorganisms and spoilage enzymes without significantly compromising sensory qualities. The technique has been pivotal in food safety management, especially for consumables susceptible to microbial contamination such as milk,...
Methods of Controlling Food Spoilage01:26

Methods of Controlling Food Spoilage

Food spoilage is caused by microbial growth or by chemical and physical changes, all of which affect the taste, texture, and safety of food.Temperature-Based PreservationRefrigeration at 0–4 °C slows microbial growth and enzyme activity, making it ideal for short-term storage. However, certain spoilage organisms—such as psychrotrophs like Listeria monocytogenes—can still proliferate at these temperatures. Freezing below -18 °C further slows biological processes by forming ice crystals, which...
Physical Methods for Controlling Microbial Growth: Temperature01:23

Physical Methods for Controlling Microbial Growth: Temperature

Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
Principles of Food Preservation01:27

Principles of Food Preservation

Food spoilage results from microbial growth, enzymatic activity, and environmental factors that gradually degrade the sensory, nutritional, and safety qualities of food. Preservation techniques aim to slow or halt these processes to extend shelf life and maintain product quality.A key concept in food microbiology is the microbial growth curve, which includes four phases: lag, exponential (log), stationary, and death. During the lag phase, bacteria adjust to their environment without significant...
Microbial Spoilage of Food01:23

Microbial Spoilage of Food

Microbial food spoilage refers to the degradation of food quality resulting from the metabolic activity of microorganisms such as bacteria, yeasts, and molds. These microbes proliferate on various food substrates depending on factors such as moisture content, nutrient availability, and storage conditions, leading to undesirable sensory and structural changes.Bacteria are primary agents of spoilage in high-moisture, nutrient-dense foods like meat, milk, and vegetables. Microbial spoilage occurs...
Microbes in the Production of Fermented Foods01:27

Microbes in the Production of Fermented Foods

Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...