Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endotoxin-induced decrease in muscle protein synthesis is associated with changes in eIF2B, eIF4E, and IGF-I.

C H Lang1, R A Frost, L S Jefferson

  • 1Departments of Cellular and Molecular Physiology, and Surgery, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA. clang@psu.edu

American Journal of Physiology. Endocrinology and Metabolism
|May 29, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cardiac myosin motor deficits are associated with left ventricular dysfunction in human ischemic heart failure.

American journal of physiology. Heart and circulatory physiology·2022
Same author

[Clinical characteristics of SLC35A2 gene variants related congenital disorders of glycosylation typeⅡ].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2020
Same author

Effects of phytonutrients alone or in combination with monensin on productivity in lactating dairy cows.

Journal of dairy science·2018
Same author

Effects of slow-release urea and rumen-protected methionine and histidine on mammalian target of rapamycin (mTOR) signaling and ubiquitin proteasome-related gene expression in skeletal muscle of dairy cows.

Journal of dairy science·2016
Same author

Studies on the meat production characteristics of Botswana goats and sheep-Part I: Sampling, methods and materials, and measurements on the live animals.

Meat science·2011
Same author

Pharmacokinetic differences in exposure to camphor after intraruminal dosing in selectively bred lines of goats.

Journal of animal science·2010

Endotoxin (LPS) administration inhibits skeletal muscle protein synthesis by reducing translational efficiency. This occurs due to impaired translation initiation, affecting eukaryotic initiation factors and insulin-like growth factor I.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Physiology

Background:

  • Sepsis-induced muscle protein synthesis inhibition is a significant clinical concern.
  • Understanding the molecular mechanisms is crucial for developing therapeutic strategies.

Purpose of the Study:

  • To investigate the mechanisms by which endotoxin (LPS) inhibits skeletal muscle protein synthesis.
  • To identify key molecular players involved in the regulation of translation initiation.

Main Methods:

  • Rats were intravenously injected with Escherichia coli LPS.
  • Muscle samples were collected at 4 and 24 hours post-injection for analysis.
  • Assessed protein synthesis rates, RNA content, translational efficiency, and eukaryotic initiation factors (eIFs).

Related Experiment Videos

Main Results:

  • LPS administration reduced skeletal muscle protein synthesis and translational efficiency at both 4 and 24 hours.
  • While eIF2alpha levels and phosphorylation remained unchanged, eIF2B activity decreased at 4 hours post-LPS.
  • LPS altered eIF4E availability by affecting its interaction with 4E-binding protein-1 (4E-BP1) and eIF4G, and decreased insulin-like growth factor I (IGF-I).

Conclusions:

  • LPS impairs skeletal muscle protein synthesis primarily by reducing translational efficiency.
  • This impairment stems from defects in translation initiation, involving altered eIF2B activity and eIF4E availability.
  • IGF-I levels correlate with protein synthesis rates and eIF4E-eIF4G association, suggesting its role in LPS-induced muscle catabolism.