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Forward with BACs: new tools for herpesvirus genomics
W Brune1, M Messerle, U H Koszinowski
1Max von Pettenkofer Institute, Department of Virology, University of Munich, Germany. wolfram@lmb.uni-muenchen.de
Trends in Genetics : TIG
|May 29, 2000
Summary
Bacterial artificial chromosome (BAC) technology now allows bacterial genetics tools to analyze complex herpesvirus genomes. This advances herpesvirus research and aids in developing new vectors and vaccines.
Area of Science:
- Virology
- Genetics
- Molecular Biology
Background:
- Herpesviruses possess large, complex genomes reflecting host adaptation.
- Traditional herpesvirus genetic analysis methods mirrored eukaryotic cell genetics.
- Limited tools existed for detailed herpesvirus genome manipulation.
Purpose of the Study:
- To highlight the impact of bacterial artificial chromosome (BAC) technology on herpesvirus genetics.
- To explore new research and development opportunities enabled by BAC technology.
Main Methods:
- Application of bacterial artificial chromosome (BAC) technology to herpesvirus genomes.
- Utilizing tools from bacterial genetics for herpesvirus analysis.
Main Results:
- Herpesvirus genomes are now accessible to bacterial genetics techniques.
- New possibilities for reverse and forward genetics in herpesviruses have emerged.
Conclusions:
- Bacterial artificial chromosome (BAC) technology has revolutionized herpesvirus genetics.
- This advancement facilitates basic research and the development of novel vectors and vaccines for herpesviruses.