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Related Experiment Videos

B-lymphocytosis factor in human plasma.

H Sakai, H Kuno-Sakai, G A Beathard

    Bollettino Dell'Istituto Sieroterapico Milanese
    |July 31, 1975
    PubMed
    Summary

    Burn patients exhibit increased B-lymphocytes during recovery. Researchers identified a plasma factor, B-lymphocytosis factor (BLF), that stimulates B-lymphocyte production and transformation, suggesting its role in regulating B-lymphocyte levels post-burn.

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    Area of Science:

    • Immunology
    • Burn Medicine

    Background:

    • Acute thermal burns trigger immune responses, including increased serum immunoglobulins and B-lymphocytosis during recovery.
    • The specific humoral factors responsible for B-lymphocytosis in burn patients remain largely undefined.

    Purpose of the Study:

    • To identify and characterize humoral factors in burn patients that induce B-lymphocytosis.
    • To investigate the role of these factors in B-lymphocyte regulation following thermal injury.

    Main Methods:

    • Plasma samples from burn patients and controls were injected into mice to assess B-lymphocyte counts.
    • Plasma fractions were isolated, and the active component, B-lymphocytosis factor (BLF), was identified.
    • In vitro lymphocyte transformation assays and solubility tests were performed on BLF.

    Main Results:

    • Plasma from burn patients significantly increased immunoglobulin-bearing cells (IgG, IgA, IgM) in mice within 3 hours.
    • Normal plasma showed no significant B-lymphocytosis activity.
    • BLF induced in vitro transformation of normal human lymphocytes and had a molecular weight near 13,400 Da.

    Conclusions:

    • A novel plasma factor, BLF, is identified in burn patients, responsible for inducing B-lymphocytosis.
    • BLF plays a role in the regulation of B-lymphocyte levels during the recovery phase of burn injuries.
    • BLF is not a lipopolysaccharide and influences lymphocyte proliferation.

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