Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple forms of human renin. Purification and characterization.

F X Galen, C Devaux, T Guyenne

    The Journal of Biological Chemistry
    |June 10, 1979
    PubMed
    Summary

    Researchers purified human renin from a tumor, identifying five glycoprotein forms with high specific activity. These tumor-derived renins share biochemical properties with kidney renin, suggesting potential therapeutic applications.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    What could explain the late emergence of COVID-19 in Africa?

    New microbes and new infections·2020
    Same author

    A21 HIV-1 sub-subtype F1 outbreak among MSM in Belgium.

    Virus evolution·2017
    Same author

    Controlled structure and density of "living" polystyrene brushes on flat silica surfaces.

    The European physical journal. E, Soft matter·2016
    Same author

    Ecological speciation in sympatric palms: 2. Pre- and post-zygotic isolation.

    Journal of evolutionary biology·2016
    Same author

    Ecological speciation in sympatric palms: 1. Gene expression, selection and pleiotropy.

    Journal of evolutionary biology·2016
    Same author

    Postoperative discomfort after outpatient anterior cruciate ligament reconstruction: a prospective comparative study.

    Orthopaedics & traumatology, surgery & research : OTSR·2015

    Area of Science:

    • Biochemistry
    • Enzymology
    • Molecular Biology

    Background:

    • Human renin is a key enzyme in the renin-angiotensin system, regulating blood pressure.
    • Tumors, particularly juxtaglomerular cell tumors, can exhibit high renin content.
    • Understanding renin's properties is crucial for diagnosing and treating renin-dependent hypertension.

    Purpose of the Study:

    • To purify and characterize human renin from a juxtaglomerular cell tumor.
    • To compare the biochemical properties of tumor-derived renin with standard kidney renin.
    • To investigate the potential heterogeneity and subunit composition of human renin.

    Main Methods:

    • Purification using gel filtration, DEAE-cellulose chromatography, and preparative isoelectric focusing.
    • Biochemical characterization including isoelectric focusing, immunoelectrophoresis, and polyacrylamide gel electrophoresis.

    Related Experiment Videos

  • Molecular weight determination via gel filtration and Fergusson plot analysis.
  • Enzyme kinetics studies (pH optimum, Km) and dissociation analysis under denaturing conditions.

    • Main Results:

    • Five distinct renin forms (isoelectric points 4.95-5.70) were purified, all identified as glycoproteins.
    • High specific activity (up to 868 Goldblatt units/mg protein) was observed for the major fractions.
    • Tumor renin exhibited identical biochemical and kinetic properties (pH optimum, Km) to standard human kidney renin.
    • Renin activity was inhibited by antibodies raised against tumor renin.
    • Under dissociating conditions, renin activity was found in fragments of Mr 20,000 and 25,000.

    Conclusions:

    • Human renin from juxtaglomerular cell tumors can be purified to homogeneity.
    • The purified tumor renin is biochemically and kinetically indistinguishable from kidney renin.
    • Tumor renin exists as multiple isoforms, potentially arising from post-translational modifications.
    • The enzyme appears to be composed of subunits, as indicated by dissociation studies.