Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple transcripts encoding lamprey gonadotropin-releasing hormone-I precursors.

K Suzuki1, R L Gamble, S A Sower

  • 1Department of Biochemistry and Molecular Biology, Rudman Hall, University of New Hampshire, Durham, New Hampshire 03824, USA.

Journal of Molecular Endocrinology
|June 1, 2000
PubMed
Summary

Researchers identified the sea lamprey gonadotropin-releasing hormone-I (GnRH-I) precursor, revealing conserved sequences and unique multiple transcripts produced by alternate splicing, suggesting an ancient gene regulation mechanism.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modulation of cellular signals by calpain.

Annals of the New York Academy of Sciences·1992
Same author

Influence of oxidative stress on induced tolerance to ischemia in gerbil hippocampal neurons.

Brain research·1992
Same author

Positive regulation of mu-calpain action by polyphosphoinositides.

The Journal of biological chemistry·1992
Same author

[Conditions for low-intensity voluntary wheel running in rats and its chronic effects on health indexes].

Nihon eiseigaku zasshi. Japanese journal of hygiene·1992
Same author

Fluorescent probe-labeled lipid microsphere uptake by human endothelial cells: a flow cytometric study.

Japanese journal of pharmacology·1992
Same author

[Antibacterial activity of rokitamycin against fresh clinical isolates].

The Japanese journal of antibiotics·1992

Area of Science:

  • Comparative genomics
  • Evolutionary endocrinology
  • Molecular biology of lampreys

Background:

  • Gonadotropin-releasing hormone (GnRH) is a key regulator of reproduction across vertebrates.
  • Understanding GnRH evolution provides insights into vertebrate origins and gene regulation.
  • Lampreys represent an ancient vertebrate lineage, offering a unique perspective on conserved biological mechanisms.

Purpose of the Study:

  • To isolate and sequence the cDNA encoding lamprey GnRH-I in the sea lamprey (Petromyzon marinus).
  • To investigate the evolutionary conservation and structural characteristics of lamprey GnRH-I.
  • To identify and characterize the transcripts and gene structure of lamprey GnRH-I.

Main Methods:

  • cDNA isolation and sequencing of lamprey prepro-gonadotropin releasing hormone-I (GnRH-I).

Related Experiment Videos

  • Bioinformatic analysis to compare sequences with other vertebrate GnRH precursors.
  • Analysis of gene structure, including intron-splicing junctions, to identify transcript variants.

    • Main Results:

    • The lamprey GnRH-I precursor shares a conserved tripartite structure with other vertebrates.
    • Lamprey GnRH-I amino acid sequence and processing sites show high conservation (60-70% identity) over 500 million years.
    • GnRH-associated peptide regions are highly divergent (<20% identity).
    • Three distinct lamprey GnRH-I transcripts (GAP49, GAP50, GAP58) were identified, arising from alternate splicing of a single gene.
    • This is the first demonstration of a GnRH gene utilizing splice sequence variants to produce multiple transcripts.

    Conclusions:

    • Lamprey GnRH-I provides insights into the early evolution of GnRH and its regulation in vertebrates.
    • The use of alternate splicing to generate multiple transcripts from a single GnRH gene may represent an ancestral gene regulatory mechanism.
    • Divergence in GnRH-associated peptides suggests differential functional roles or evolutionary pressures in lampreys.