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Related Experiment Videos

The active E rosette test: correlation with delayed cutaneous hypersensitivity.

P J Felsburg, R Edelman, R H Bilman

    Journal of Immunology (Baltimore, Md. : 1950)
    |April 1, 1976
    PubMed
    Summary
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    A distinct group of peripheral blood T lymphocytes, identified by rapid rosette formation with sheep erythrocytes (A-RFC), showed a significant increase in individuals with positive delayed cutaneous hypersensitivity (DCH) reactions, indicating their active role in immune responses.

    Area of Science:

    • Immunology
    • Cellular Immunology
    • T cell subpopulations

    Background:

    • Peripheral blood T lymphocytes play a crucial role in cell-mediated immunity.
    • Delayed cutaneous hypersensitivity (DCH) is a key indicator of cellular immune response.
    • Identifying distinct T cell subpopulations is vital for understanding immune function.

    Purpose of the Study:

    • To investigate the dynamic changes in T lymphocyte subpopulations following microbial antigen skin testing.
    • To correlate the activity of specific T cell subsets with the development of DCH reactions.
    • To identify a potentially distinct and immunologically active T cell subpopulation.

    Main Methods:

    • Measurement of T-lymphocyte subpopulations using rapid (5-min) and standard (60-min) rosette formation with sheep erythrocytes (A-RFC).

    Related Experiment Videos

  • Assessment of delayed cutaneous hypersensitivity (DCH) reactions after skin testing with microbial antigens.
  • Quantification of B cells via surface immunoglobulin immunofluorescence.

    • Main Results:

    • A significant increase in rapid rosette-forming T cells (A-RFC) was observed exclusively in individuals exhibiting positive DCH reactions.
    • Skin test non-responders did not show a significant rise in A-RFC levels.
    • Total T cells (60-min rosette formation) and B cells did not exhibit consistent changes correlating with DCH response.

    Conclusions:

    • The rapid rosette-forming T cell (A-RFC) subpopulation represents a biologically distinct and active component of the peripheral blood T lymphocytes.
    • A-RFC levels serve as a sensitive indicator of in vivo cellular immune activity, closely paralleling DCH responses.
    • These findings highlight the immunological significance of the A-RFC subpopulation in cellular immunity.