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Updated: Aug 19, 2026

Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer
Published on: November 4, 2009
Interference of thimerosal in the bicinchoninic acid protein microassay
1College of Pharmacy, Nova Southeastern University, Ft. Lauderdale, FL 33328, USA.
Abstract:
In this investigation the total amount of protein present in the three influenza vaccine formulations Fluvirin, Fluzone and Flushield have been tested where thimerosal is present as a preservative. Interaction of thimerosal with protein has been confirmed by analysing ovalbumin as a control against ovalbumin-thimerosal solution. The results indicate that interference of thimerosal can be overcame for the quantitative assay of proteins by BCA technique with the good reproducibility. The present study identified also the degree of interference of thimerosal in the protein formulations and development of a method to nullify the interference by removing thimerosal.
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