[Membrane model in the presentation of surface immunoglobulin on human lymphocytes with peroxidase cabeled...

For the demonstration of B-lymphocytes the direct immune peroxydase technique with anti-human IgM was used. In contrast to the biochemical and immunological controls in 11.1% of 30 normal persons positive membrane reactions could be proved without doubt. A certain distinction from other white blood cells and falsely positive contrast reactions is possible by means of counterstain with genuine nuclear red and after contrastation with osmium tetroxyde. The method described has a large number of advantages compared with other methods of the determination of B-cells, which appear usable for the clinician above all on account of the relevance to practice.