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Published on: November 1, 2012
Interaction of DNA with [Cr(Schiff base)(H(2)O)(2)]ClO(4)
R Vijayalakshmi1, M Kanthimathi, V Subramanian
1Chemical Laboratory, Central Leather Research Institute, Adyar, 600 020, Chennai, India.
Biochimica Et Biophysica Acta
|June 1, 2000
Summary
Chromium(III) Schiff base complexes bind to calf thymus DNA through a non-intercalative major groove binding mode. These complexes show potential for DNA cleavage and interaction, influencing DNA structure and properties.
Area of Science:
- Coordination Chemistry
- Biophysical Chemistry
- Molecular Biology
Background:
- Schiff base complexes are versatile ligands in coordination chemistry.
- Chromium complexes have shown potential biological activities.
- Understanding metal-DNA interactions is crucial for developing novel therapeutic agents.
Purpose of the Study:
- To investigate the binding interactions of chromium(III) Schiff base complexes with calf thymus DNA.
- To elucidate the mode of DNA binding and the effects of these complexes on DNA structure.
- To assess the potential of these complexes in DNA cleavage.
Main Methods:
- UV-Vis absorption spectroscopy
- Fluorescence spectroscopy
- Circular dichroism (CD) spectroscopy
- Melting temperature studies
- Viscosity measurements
- Plasmid DNA cleavage assays
Main Results:
- Chromium(III) complexes exhibited hyperchromicity, red shift, and fluorescence enhancement upon binding to DNA.
- Binding studies revealed non-intercalative interactions, with a preferred major groove binding mode.
- The binding constants were determined, and one complex showed a binding stoichiometry of 10 base pairs per complex.
- Complexes induced single-strand cleavage in plasmid DNA.
Conclusions:
- The Schiff base chromium(III) complexes interact with DNA via a non-intercalative, major groove binding mechanism.
- These complexes can alter DNA properties and induce DNA cleavage, suggesting potential applications in medicinal chemistry.
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