Related Experiment Video
Updated: Aug 1, 2026

Induction and Monitoring of Active Delayed Type Hypersensitivity (DTH) in Rats
Published on: July 19, 2007
Interaction of 6,6'-dithiodinicotinic acid with rat thymocytes pretreated with thiol compounds
Abstract:
The number of sulfhydryl groups in the membranes of rat thymocytes were determined, expressed in terms of concentration of 6-mercaptonicotinic acid produced by reduction of 6,6'-dithiodinicotinic acid. The influence of incubation of thymocytes in solutions of L-cysteine, reduced glutathione and dithiothreitol on the number of -SH groups was studied. About twofold higher degree of reduction of 6-6'dithiodinicotinic acid was observed in the reaction with thymocytes incubated previously in 2 mM solution of L-cysteine in comparison with controls. Incubation of thymocytes with dithiothreitol and gluthathione had no effect on the number of -SH groups.
Related Concept Videos
Preparation and Reactions of Thiols
Hypersensitivity Reactions: Delayed Hypersensitivity Reactions

