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Oxalate-degrading Enterococcus faecalis.

S Hokama1, Y Honma, C Toma

  • 1Department of Urology, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan.

Microbiology and Immunology
|June 1, 2000
PubMed
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Researchers isolated oxalate-degrading Enterococcus faecalis from human stools. This facultative anaerobic bacterium requires specific conditions to maintain its oxalate-degrading ability, identified through protein analysis.

Area of Science:

  • Microbiology
  • Gastroenterology
  • Biochemistry

Background:

  • Oxalate degradation is crucial for preventing calcium oxalate urolithiasis.
  • Oxalate-degrading bacteria are primarily known as obligate anaerobes, such as Oxalobacter formigenes.
  • The isolation of oxalate-degrading facultative anaerobic bacteria from humans has not been previously reported.

Purpose of the Study:

  • To isolate and characterize oxalate-degrading bacteria from human stools.
  • To investigate the proteins involved in oxalate degradation in Enterococcus faecalis.
  • To determine if these proteins are homologous to known oxalate-degrading enzymes.

Main Methods:

  • Isolation of bacteria from human stools under anaerobic conditions.
  • Culturing bacteria in a poor nutritional environment with repeated subculturing.

Related Experiment Videos

  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to analyze protein production.
  • Western blotting using antibodies against known oxalate-degrading enzymes from Oxalobacter formigenes.

    • Main Results:

    • An oxalate-degrading Enterococcus faecalis strain was successfully isolated from human stools.
    • This E. faecalis strain produced three unique proteins (65, 48, and 40 kDa) compared to non-degrading strains.
    • Antibodies against oxalyl-coenzyme A decarboxylase (65 kDa) and formyl-coenzyme A transferase (48 kDa) from O. formigenes reacted with the corresponding proteins in E. faecalis.
    • The oxalate-degrading ability of E. faecalis was maintained under specific nutritional and subculturing conditions.

    Conclusions:

    • This study reports the first isolation of oxalate-degrading facultative anaerobic bacteria (Enterococcus faecalis) from humans.
    • The identified E. faecalis utilizes enzymatic pathways similar to O. formigenes for oxalate degradation, involving proteins of 65 and 48 kDa.
    • These findings suggest a potential role for E. faecalis in oxalate metabolism within the human gut microbiome.