Related Experiment Video

Updated: Aug 14, 2026

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH
07:54

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

Published on: August 19, 2014

p300/CBP-dependent and -independent transcriptional interference between NF-kappaB RelA and p53

A Ikeda1, X Sun, Y Li

  • 1Department of Molecular Pathology, Cancer Research Institute, Kanazawa University, 13-1 Takaramachi, Kanazawa, 920-0934, Japan.

Biochemical and Biophysical Research Communications
|June 2, 2000
PubMed

Insights

The tumor suppressor p53 and transcription factor NF-kappaB RelA directly interact, inhibiting each other's activity. This interaction is crucial for cellular responses to genotoxic stress.

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Genetics

Background:

  • p53 and NF-kappaB RelA are activated by genotoxic agents.
  • These factors mutually suppress transcriptional activity, potentially via coactivator competition (CBP/p300).
  • Previous findings suggested incomplete restoration of p53 activity by coactivator overexpression and a p53 mutant's inability to suppress RelA.

Purpose of the Study:

  • To investigate the mechanism of mutual transcriptional suppression between p53 and NF-kappaB RelA.
  • To determine if direct interaction occurs between p53 and RelA.
  • To assess the physiological importance of this interaction in genotoxic stress response.

Main Methods:

  • In vivo and in vitro interaction assays.
  • Analysis of transcriptional activity in RelA knockout mouse cells.
  • Mutational analysis of p53 interaction domains.

Main Results:

  • p53 and RelA were found to directly interact both in vivo and in vitro.
  • Direct interaction is mediated by their dimerization/tetramerization domains.
  • Cells lacking RelA exhibited enhanced p53 transcriptional activity.

Conclusions:

  • Direct physical interaction between p53 and RelA contributes to their mutual transcriptional inhibition.
  • This interaction, mediated by specific domains, plays a significant physiological role in cellular responses to genotoxic stress.

More Related Videos

Yeast As a Chassis for Developing Functional Assays to Study Human P5314:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence04:56

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

Related Concept Videos

Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...