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Related Experiment Videos

Suppressor cells: dependence on assay conditions for functional activity.

D D Eardley, M O Staskawicz, R K Gershon

    The Journal of Experimental Medicine
    |May 1, 1976
    PubMed
    Summary

    Spleen cells educated with sheep red blood cells (SRBC) can either suppress or enhance immune responses. Their effect depends on the target cell population

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    Area of Science:

    • Immunology
    • Cellular Immunology

    Background:

    • The immune system relies on complex regulatory networks.
    • Spleen cells educated in vitro can modulate immune responses.

    Purpose of the Study:

    • To investigate how educated spleen cells affect plaque-forming cell responses.
    • To determine the conditions under which educated cells suppress or augment immune responses.

    Main Methods:

    • Mishell-Dutton assay using spleen cells and sheep red blood cells (SRBC).
    • Manipulation of assay conditions including cell numbers, antigen dose, and culture environment (rocking vs. stationary).

    Main Results:

    • Educated spleen cells suppressed plaque-forming cell response at optimal SRBC doses.
    • Educated cells augmented responses with suboptimal spleen cell numbers or suboptimal SRBC doses.
    • Suppression was reinstated by adding normal T cells to B cell cultures.
    • Stationary culture conditions led to augmentation instead of suppression.

    Conclusions:

    • The regulatory effect of educated cells is context-dependent.
    • Educated cells augment immune responses when the target population's activity is low.
    • Educated cells suppress immune responses when the target population's activity is high, supporting a regulatory feedback hypothesis.

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