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Related Experiment Videos

Misidentifying helicobacters: the Helicobacter cinaedi example.

P Vandamme1, C S Harrington, K Jalava

  • 1Laboratorium voor Microbiologie, Faculteit Wetenschappen, Universiteit Gent, Ghent, Belgium. Peter.Vandamme@rug.ac.be

Journal of Clinical Microbiology
|June 2, 2000
PubMed
Summary
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Whole-cell protein electrophoresis and DNA-DNA hybridization accurately identified Helicobacter species, revealing 16S rRNA gene sequencing can be misleading. Helicobacter westmeadii is now recognized as Helicobacter cinaedi.

Area of Science:

  • Microbiology
  • Bacteriology
  • Genomics

Background:

  • Accurate identification of bacterial species is crucial for understanding their role in health and disease.
  • Traditional and molecular methods are used for bacterial identification, but their limitations require continuous evaluation.

Purpose of the Study:

  • To accurately identify 14 putative Helicobacter species using a polyphasic approach.
  • To evaluate the reliability of 16S ribosomal DNA (rDNA) gene sequencing for Helicobacter species identification.
  • To clarify the taxonomic status of Helicobacter westmeadii and related strains.

Main Methods:

  • Whole-cell protein electrophoresis and a panel of 64 biochemical tests for phenotypic characterization.
  • DNA-DNA hybridization experiments for genotypic confirmation.

Related Experiment Videos

  • Analysis of near-complete 16S ribosomal DNA gene sequences.

    • Main Results:

    • Protein electrophoresis and biochemical tests, validated by DNA-DNA hybridization, provided reliable species-level identification.
    • "Helicobacter westmeadii" was confirmed as a junior synonym of Helicobacter cinaedi.
    • Helicobacter sp. strain Mainz was identified as Helicobacter cinaedi, highlighting species-level discrepancies with 16S rRNA gene sequencing.

    Conclusions:

    • Near-complete 16S rDNA sequence comparison can be misleading for Helicobacter species identification.
    • Helicobacter cinaedi is a widely distributed species found in various animal reservoirs.
    • Developing robust identification strategies is essential for understanding the significance of Helicobacter cinaedi.