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New polymers forming aqueous two-phase polymer systems.

N Pietruszka1, I Y Galaev, A Kumar

  • 1Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, Lund, S-221 00, Sweden.

Biotechnology Progress
|June 3, 2000
PubMed
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A novel acrylamide-modified starch polymer effectively forms aqueous two-phase systems (ATPS) for protein separation. This polymer, combined with a metal-loaded copolymer, achieved 75% yield in purifying alpha-amylase inhibitor.

Area of Science:

  • Polymer Chemistry
  • Bioseparations
  • Protein Purification

Background:

  • Aqueous two-phase systems (ATPS) are valuable for protein separation.
  • Developing novel polymers for ATPS is crucial for efficient bioprocessing.

Purpose of the Study:

  • To develop and evaluate a starch-modified acrylamide polymer for ATPS.
  • To explore the use of this polymer with a thermoprecipitating copolymer for metal affinity partitioning and protein purification.

Main Methods:

  • Starch modification via partial hydrolysis and acrylamide grafting.
  • Formation of ATPS with poly(ethylene glycol) and a thermoprecipitating copolymer (poly-VI/VCL).
  • Metal affinity partitioning using Cu(II)-loaded poly-VI/VCL.

Main Results:

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  • Modified starch successfully formed ATPS with poly(ethylene glycol) and poly-VI/VCL.
  • Cu(II)-loaded poly-VI/VCL enabled metal affinity partitioning.
  • Purification of alpha-amylase inhibitor from wheat meal achieved with 75% yield.

Conclusions:

  • Acrylamide-modified starch is a promising polymer for ATPS applications.
  • The developed ATPS method is effective for protein purification, specifically alpha-amylase inhibitor.
  • The combination of modified starch and metal-loaded copolymer offers a versatile platform for bioseparations.