Ontogeny and localization of TGF-beta type I receptor expression during lung development

¹Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA. zhaoyun@duke.edu

American Journal of Physiology. Lung Cellular and Molecular Physiology

|June 3, 2000

Abstract:

Transforming growth factor (TGF)-beta is a family of multifunctional cytokines controlling cell growth, differentiation, and extracellular matrix deposition in the lung. The biological effects of TGF-beta are mediated by type I (TbetaR-I) and II (TbetaR-II) receptors. Our previous studies show that the expression of TbetaR-II is highly regulated in a spatial and temporal fashion during lung development. In the present studies, we investigated the temporal-spatial pattern and cellular expression of TbetaR-I during lung development. The expression level of TbetaR-I mRNA in rat lung at different embryonic and postnatal stages was analyzed by Northern blotting. TbetaR-I mRNA was expressed in fetal rat lungs in early development and then decreased as development proceeded. The localization of TbetaR-I in fetal and postnatal rat lung tissues was investigated by using in situ hybridization performed with an antisense RNA probe. TbetaR-I mRNA was present in the mesenchyme and epithelium of gestational day 14 rat lungs. An intense TbetaR-I signal was observed in the epithelial lining of the developing bronchi. In gestational day 16 lungs, the expression of TbetaR-I mRNA was increased in the mesenchymal tissue. The epithelium in both the distal and proximal bronchioles showed a similar level of TbetaR-I expression. In postnatal lungs, TbetaR-I mRNA was detected in parenchymal tissues and blood vessels. We further studied the expression of TbetaR-I in cultured rat lung cells. TbetaR-I was expressed by cultured rat lung fibroblasts, microvascular endothelial cells, and alveolar epithelial cells. These studies demonstrate a differential regulation and localization of TbetaR-I that is different from that of TbetaR-II during lung development. TbetaR-I, TbetaR-II, and TGF-beta isoforms exhibit distinct but overlapping patterns of expression during lung development. This implies a distinct role for TbetaR-I in mediating TGF-beta signal transduction during lung development.

Related Experiment Videos

Ontogeny and localization of TGF-beta type I receptor expression during lung development

Related Concept Videos

First evidence of legacy and emerging per- and polyfluoroalkyl substances (PFAS) in the follicular fluid of a cohort of North Carolina in vitro fertilization (IVF) patients.

Alterations to Placental Glucocorticoid Receptor Expression with Alcohol Consumption.

A Critical Review of State-of-the-Art and Emerging Approaches to Identify Fracking-Derived Gases and Associated Contaminants in Aquifers.

Suppression of the CD8 T cell response by human papillomavirus type 16 E7 occurs in Langerhans cell-depleted mice.

cAMP-Response Element-Binding 3-Like Protein 1 (CREB3L1) is Required for Decidualization and its Expression is Decreased in Women with Endometriosis.

Altered expression of microRNA-451 in eutopic endometrium of baboons (Papio anubis) with endometriosis.

MicroRNAs in IPF and Lung Cancer: Convergent and Divergent Mechanisms: a systematic review.

Mechanical and Chemical Power and Mortality in Acute Respiratory Distress Syndrome: An Exploratory Analysis of Six Patient Cohorts.

Testosterone alleviates inflammation but increases the methacholine response in male mice with allergic lung inflammation.

Conversations in Collagen: Multicellular Crosstalk Shapes the Fibrotic Niche in Pulmonary Fibrosis.

Central Cholinergic Modulation of the Cough Reflex: Suppressive Effects of Donepezil in rabbits.

Efficient AI-supported quantification of network structures in SEM imaged human mucus: A detailed protocol.

Extravascular distribution of factor IX-review of experimental and clinical evidence, and relevance for hemophilia B replacement therapy.

Dose-response relationship of pre-ICU corticosteroids and influenza-associated pulmonary aspergillosis: a multicenter target trial emulation.

Simultaneous targeting of IL-10 and PD-(L)1 pathways with a bispecific anti-PD-L1/IL-10-trap antibody enhances T cell responses.

SARS-CoV-2-induced IgG repertoires shape T cell responses, microRNA regulation, and autoreactivity according to disease severity.

Multi-target Therapy with Rituximab plus Mycophenolate Mofetil versus Rituximab Monotherapy in Systemic Sclerosis-Associated Interstitial Lung Disease.

Upadacitinib effectively suppresses IL13RA2 expression in primary human intestinal fibroblasts derived from Crohn's disease tissue.

Related Experiment Videos

Ontogeny and localization of TGF-beta type I receptor expression during lung development

Abstract:

Related Concept Videos

Related Articles