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beta-lactamase expression in Plesiomonas shigelloides.

M B Avison1, P M Bennett, T R Walsh

  • 1Bristol Centre for Antimicrobial Research and Evaluation (BCARE), Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK. matthewb.avison@bris.ac.uk

The Journal of Antimicrobial Chemotherapy
|June 6, 2000
PubMed
Summary

Plesiomonas shigelloides produces beta-lactamases, enzymes conferring antibiotic resistance. This study found chromosomally encoded beta-lactamases in clinical and environmental isolates, highlighting potential resistance reservoirs.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Antimicrobial Resistance

Background:

  • Plesiomonas shigelloides is an opportunistic pathogen found in both clinical and environmental settings.
  • Beta-lactamases are enzymes produced by bacteria that confer resistance to beta-lactam antibiotics, a critical class of drugs.

Purpose of the Study:

  • To investigate the production and characteristics of beta-lactamases in Plesiomonas shigelloides.
  • To determine the prevalence of beta-lactamase-mediated antibiotic resistance in clinical and environmental isolates.

Main Methods:

  • Analysis of beta-lactamase production in 11 clinical and 9 environmental Plesiomonas shigelloides isolates.
  • Characterization of beta-lactamase types, including isoelectric points (pI), and assessment of antibiotic resistance profiles.

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Main Results:

  • Fifty percent of isolates produced detectable chromosomally encoded, non-inducible beta-lactamases.
  • Isolates exhibited low-level resistance to ampicillin and higher resistance to carbenicillin, but remained susceptible to cephalosporins and meropenem.
  • Specific beta-lactamase types, including class 2c carbenicillinase and class 2d oxacillinase, were identified in clinical isolates, while environmental isolates showed diverse penicillinases.

Conclusions:

  • Plesiomonas shigelloides possesses a reservoir of heterogeneous beta-lactamase genes, particularly in environmental strains.
  • The presence of these enzymes contributes to the antibiotic resistance profile of this species, necessitating further surveillance.