Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Self-amplifying vectors for gene delivery.

Wolff1, Herweijer

  • 1Waisman Center, University of Wisconsin-Madison, 1500 Highland Avenue, Madison, WI 53705, USA

Advanced Drug Delivery Reviews
|August 11, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

On a case of cord hematoma.

Gynecologie et obstetrique·2010
Same author

A case of Brocq-Duhring's disease cured by homeopathy.

L' Homeopathie francaise·2010
Same author

On the presence of Trichomonas in the urine of a pregnant woman.

Gynecologie et obstetrique·2010
Same author

On a case of cord hematoma.

Journal de medecine de Bordeaux et du Sud-Ouest·2010
Same author

On the presence of Trichomonas in the urine of a pregnant woman.

Journal de medecine de Bordeaux et du Sud-Ouest·2010
Same author

Optimal process design for the manufacture of transdermal drug delivery systems.

Pharmaceutical science & technology today·2000

Self-amplifying expression vectors enhance gene transfer efficiency by utilizing alphavirus replication machinery for high transgene expression. These vectors show improved cell spread, making them effective for in vivo applications.

Area of Science:

  • Molecular Biology
  • Virology
  • Biotechnology

Background:

  • Gene transfer methods often exhibit low in vivo efficiency.
  • Achieving high expression levels in transduced cells is crucial for therapeutic applications.

Purpose of the Study:

  • To evaluate self-amplifying expression vectors for enhanced gene transfer and expression.
  • To investigate the potential of alphavirus-based vectors for improved in vivo gene delivery.

Main Methods:

  • Utilizing self-amplifying expression vectors derived from Sindbis virus or Semliki Forest virus.
  • Replacing viral capsid protein coding sequences with a gene of interest.
  • Introducing vectors as RNA, DNA, or virions into target cells.

Main Results:

Related Experiment Videos

  • Self-amplifying vectors lead to significantly higher transgene expression levels compared to conventional methods.
  • Recombinant genome replication by viral proteins amplifies mRNA production.
  • Vectors demonstrate enhanced spread within large cells like muscle and neurons.

Conclusions:

  • Self-amplifying expression vectors offer a promising strategy to overcome low gene transfer efficiency.
  • These vectors enable robust and widespread transgene expression, particularly beneficial for in vivo applications.
  • The versatility in vector format (RNA, DNA, virions) adds to their applicability.