Lipid fusion in oligonucleotide and gene delivery with cationic lipids

Delivery of oligonucleotides and genes to their intracellular targets is a prerequisite for their successful use in medical therapy. Cationic liposomes are among the most commonly used and promising delivery systems for oligonucleotides and genes. Lipid fusion plays an important role in the cationic liposome-mediated delivery of these compounds. Fusion is involved in the complex formation between the nucleotides and the lipids, in the interactions between extracellular materials with the complexes, as well as in the intracellular trafficking of the delivery system and its load. Since lipid fusion is such a crucial factor in polynucleotide delivery, its controlled use is important for the success in oligonucleotide and DNA delivery. In this article we are reviewing the current knowledge on lipid fusion phenomena associated with the delivery of oligonucleotides and genes.