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Related Experiment Videos

Electropermeabilization of cell membranes.

Teissié1, Eynard, Gabriel

  • 1IPBS UPR 9062 CNRS, 118 Route de Narbonne, 31062 Toulouse, France

Advanced Drug Delivery Reviews
|June 6, 2000
PubMed
Summary
This summary is machine-generated.

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Applying intense electric fields to cells causes temporary membrane alterations, allowing molecule exchange and drug loading. While lipids are the primary target, proteins are also affected, but cell viability is preserved through metabolic recovery.

Area of Science:

  • Cellular Biophysics
  • Membrane Biology
  • Drug Delivery

Background:

  • Electric fields can alter cell membrane potential.
  • High-intensity electric pulses can induce temporary membrane alterations.
  • The molecular mechanisms of electric field-induced membrane changes are not fully understood.

Purpose of the Study:

  • To investigate the effects of electric field pulses on cell membranes.
  • To understand the molecular targets and consequences of electric field exposure.
  • To explore the potential for drug delivery using electric field-induced permeabilization.

Main Methods:

  • Application of short-lived, overcritical intensity electric field pulses to cells.
  • Observation of membrane potential modulation and local membrane alterations.

Related Experiment Videos

  • Analysis of molecular exchange across the permeabilized membrane.

    • Main Results:

    • Electric fields induce position-dependent changes in membrane potential.
    • Induced membrane alterations allow free exchange of hydrophilic molecules and leakage of metabolites.
    • Lipids are primary targets, but membrane proteins are also affected; polar drugs can be loaded into the cytoplasm.
    • The permeabilized state is long-lived, with cell metabolism critical for recovery.
    • Cell viability is preserved despite membrane alterations.

    Conclusions:

    • Electric field pulses induce a reversible, permeabilized state in cell membranes.
    • This phenomenon offers a potential method for intracellular drug delivery.
    • Cellular metabolism plays a key role in resealing the membrane and maintaining viability.