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Haematopoietic progenitors from umbilical cord blood
A M Todaro1, C Pafumi, G Pernicone
1Institute of General Pathology, University of Catania, Italy.
Blood Purification
|June 6, 2000
Abstract
Background/Aim:
The aim of this study is to improve the obstetrician-based cord blood collection system and an efficient recovery of CD34+ haematopoietic progenitor stem cells.
Methods:
CD34+ cells were purified from total blood using a positive selection enrichment method, called Mini-Macs.
Results:
The final yield of CD34+ cells we obtained was 10(4) cells/ml, with a CD34+ purity of 99%.
Conclusion:
Our results confirm that, by using this method, it is possible to get a significant stem cell number, thus improving transplanting both peripheral stem cells and umbilical cord ones.