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Updated: Jul 27, 2026

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
Published on: December 17, 2013
An estimation of the frequency of precursor cells which generate cytotoxic lymphocytes
Abstract:
The cell-mediated immune response has been generated in vitro with a polyacrylamide culture system which allows the segregation of foci (clones?) of cytotoxic lymphocytes. Using the method of limiting dilutions, the frequency of precursor cells in CBA spleen cells able to generate a cytotoxic response against DBA mastocytoma is estimated at 1 per 1,700 cells.
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