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Related Experiment Videos

Prophage induction and inactivation by UV light.

B J Barnhart, S H Cox, J H Jett

    Journal of Virology
    |June 1, 1976
    PubMed
    Summary
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    UV light induces prophage in Haemophilus influenzae when a pyrimidine dimer is stabilized within the prophage genome. DNA repair mechanisms reduce this induction probability by removing dimers.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Bacteriology

    Background:

    • Bacterial viruses (bacteriophages) can integrate into the host genome.
    • UV irradiation is a known inducer of prophage, leading to viral replication.
    • DNA repair mechanisms play a crucial role in cellular response to damage.

    Purpose of the Study:

    • To investigate the role of pyrimidine dimers in prophage induction.
    • To understand the mechanism of UV-induced prophage activation in Haemophilus influenzae.
    • To explore the influence of host DNA repair systems on prophage inducibility.

    Main Methods:

    • Analysis of induction curves in UV-irradiated Haemophilus influenzae lysogens.
    • Distribution analysis of pyrimidine dimers in repair-deficient lysogens.

    Related Experiment Videos

  • Correlation studies between dimer presence and prophage induction events.

    • Main Results:

    • One pyrimidine dimer per prophage-sized DNA segment is required for preinduction.
    • Direct prophage induction correlates with dimer stabilization within the prophage genome.
    • Host excision-repair systems decrease the likelihood of dimer stabilization for induction.

    Conclusions:

    • Pyrimidine dimer formation and stabilization are critical for direct prophage induction.
    • Host DNA repair pathways modulate the efficiency of prophage induction.
    • The presence of a defective prophage can affect the inducibility of other prophages.