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Related Experiment Videos

NuMA: a nuclear protein involved in mitotic centrosome function.

C Zeng1

  • 1Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030, USA. czeng@bcm.tmc.edu

Microscopy Research and Technique
|June 8, 2000
PubMed
Summary

Nuclear mitotic apparatus protein (NuMA) is crucial for organizing and stabilizing mitotic spindle poles. A newly discovered NuMA-motor complex binds microtubules, essential for spindle organization during cell division.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Nuclear mitotic apparatus protein (NuMA) is an abundant 240 kDa protein.
  • NuMA binds microtubules (MTs) via its carboxyl-terminal region and can form filamentous polymers.
  • NuMA localizes to the nucleus during interphase and redistributes to centrosomes during mitosis.

Purpose of the Study:

  • To review the structural features and mitotic functions of NuMA.
  • To emphasize the role of the NuMA-motor complex in spindle organization.
  • To explore the cell cycle-dependent regulation of NuMA.

Main Methods:

  • Literature review of existing data on NuMA structure and function.
  • Analysis of NuMA's interaction with MTs, cytoplasmic dynein, and dynactin.

Related Experiment Videos

  • Examination of NuMA's cell cycle-dependent localization and regulation by phosphorylation.

    • Main Results:

    • NuMA is essential for organizing and stabilizing spindle poles from early mitosis to anaphase.
    • A NuMA-motor complex involving dynein and dynactin binds MTs at centrosomes, tethering them to spindle poles.
    • NuMA's function is regulated by phosphorylation and p34/CDC2 activity.

    Conclusions:

    • NuMA plays a critical role in mitotic spindle organization through its interaction with MTs and motor proteins.
    • The NuMA-motor complex is key to converging and tethering spindle MT ends to poles.
    • NuMA may represent a class of proteins with nuclear sequestration during interphase and mitotic function.