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Movement of histones in chromatin induced by shearing

D Doenecke, B J McCarthy

    European Journal of Biochemistry
    |May 1, 1976
    PubMed

    Insights

    Histones move along DNA during chromatin preparation. This study used DNA methylation to track histone movement, revealing their lateral displacement relative to DNA sites.

    Area of Science:

    • Molecular Biology
    • Epigenetics
    • Biochemistry

    Background:

    • Chromatin structure is dynamic, with histones potentially repositioning along DNA.
    • Understanding histone mobility is crucial for comprehending DNA accessibility and gene regulation.

    Purpose of the Study:

    • To investigate the lateral movement of histones during chromatin manipulation and fragmentation.
    • To develop a method for tracking histone positions relative to specific DNA sequences.

    Main Methods:

    • Utilized restriction modification methylases from Haemophilus influenzae for DNA methylation.
    • Employed poly(D-lysine) titration and pronase digestion for chromatin preparation.
    • Analyzed DNA-poly(D-lysine) complexes using cesium chloride density gradients.

    Main Results:

    • Specific DNA sites were methylated by restriction enzymes.
    • Comparison of radioactivity in free DNA versus DNA-poly(D-lysine) complexes indicated histone displacement.
    • Demonstrated lateral movement of histones relative to methylated DNA sites.

    Conclusions:

    • Histone lateral movement occurs during chromatin manipulation and fragmentation.
    • DNA methylation serves as a reliable marker for tracking histone repositioning.
    • Findings provide insights into the dynamic nature of chromatin organization.

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