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Examination of N-hydroxylation as a prerequisite mechanism of nitric oxide synthase inactivation
1Department of Pharmaceutics, School of Pharmacy, University at Buffalo, State University of New York, 14260-1200, USA.
Bioorganic & Medicinal Chemistry Letters
|June 8, 2000
Abstract:
L-N5-(1-Hydroxyiminoethyl)-ornithine (L-NHIO) and L-N6-(1-hydroxyiminoethyl)-lysine (L-NHIL) were synthesized and tested as potential intermediates in the mechanism-based inactivation of nitric oxide synthase (NOS) by L-N5-iminoethylornithine (L-NIO) and L-N6-iminoethyllysine (L-NIL). Although these compounds were determined to be competitive inhibitors, mechanism-based inactivation was not observed.