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Mouse lymphotoxin.

G Trivers, D Braungart, E J Leonard

    Journal of Immunology (Baltimore, Md. : 1950)
    |July 1, 1976
    PubMed
    Summary

    Phytohemagglutinin (PHA) stimulated mouse spleen cells produce lymphotoxin (LT). This cytotoxic protein, with a molecular weight of 41,000 daltons, is stable under specific storage conditions and has a pH range of 4.4-4.8.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Lymphotoxin (LT) is a key cytokine involved in immune responses.
    • Understanding LT's properties is crucial for its therapeutic and research applications.

    Purpose of the Study:

    • To characterize the properties of lymphotoxin produced by C3H mouse spleen cells stimulated with phytohemagglutinin (PHA).
    • To determine the molecular weight, stability, and isoelectric point of mouse LT.

    Main Methods:

    • C3H mouse spleen cells were cultured with PHA to induce LT production.
    • Cytotoxicity assays were performed using tritiated thymidine-labeled syngeneic target cells.
    • LT stability was assessed after lyophilization, cold storage, and heat treatment.
    • Molecular weight was estimated using G-200 Sephadex chromatography.
    • Isoelectric focusing was used to determine the isoelectric point.

    Main Results:

    • PHA stimulation induced LT production in mouse spleen cells.
    • LT exhibited stability after lyophilization and prolonged cold storage (4°C).
    • LT activity decreased upon heating at 56°C.
    • The molecular weight of mouse LT was determined to be approximately 41,000 daltons.
    • Isoelectric focusing revealed LT peaks with a pH range of 4.4–4.8.

    Conclusions:

    • Mouse spleen cell-derived LT is a relatively stable protein with a defined molecular weight and isoelectric point.
    • These characterized properties provide a foundation for further investigation and potential utilization of mouse LT.

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