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Updated: Aug 10, 2026

Imaging the Human Immunological Synapse
Published on: December 26, 2019
A functional role for interleukin (IL)-4-driven cyclic amp accumulation in human b lymphocytes
C E McKay1, E L Hewitt, B W Ozanne
1Division of Biochemistry & Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, Scotland, G12 8QQ.
Abstract:
Interleukin-4 (IL-4) regulates the expression of the 55-kDa alpha-subunit (CD25) of the IL-2 receptor complex in human B lymphocytes. This report suggests that the cAMP/protein kinase A (PKA) component of the IL-4 receptor signalling programme in human tonsillar B cells has a functionally important role in regulating expression of the CD25 gene by attenuating activity of a protein binding to a potent negative regulatory element (NRE) in the CD25 promoter; this effect can be mimicked by agents that elevate cAMP and blocked by inhibitors of PKA but not protein kinase C (PKC). In a B-cell line that fails to elevate cAMP, attenuate NRE-binding protein (NRE-BP) activity or express CD25 following IL-4 treatment, stimulation of cAMP accumulation by forskolin facilitates IL-4-mediated induction of both the endogenous gene and an exogenous reporter gene under the control of a minimal promoter/enhancer fragment of the CD25 gene.
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