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Comparison of non-viral transfection methods in melanoma cell primary cultures
B Micka1, B Trojaneck, S Niemitz
1Department of Medicine, Rheinische Friedrich Wilhelms Universität, Bonn, Germany.
Cytokine
|June 14, 2000
Abstract:
Melanoma primary cultures were transiently transfected via electroporation and lipofection for comparison. Transfection efficiency was superior with electroporation (58+/-9%) as compared to lipofection (23+/-9%) as determined by enhanced green fluorescent plasmid (EGFP) transfection. Secretion of IL-2 persisted for up to 3 weeks after electroporation. The increase in sensitivity against immunologic effector cells by transfection with IL-2 was not significant. Our results show the feasibility of a gene transfer into primary human melanoma cells, different from retroviral transduction.